The Chinese name of Tris HCl is trimethylol aminomethane hydrochloride, CAS No. 1185-53-1, with a buffer range of 7.5-8.5. It is a neutral buffer, but it itself contains weak alkalinity. It is often used in various biochemical experiments such as molecular biology and cell biology. Its conventional property is white powder material. When it is used, distilled water is added for configuration. After being adjusted to the required pH value, it can be stored and used after high-pressure sterilization. It is worth noting that if the solution is to be stored for a long time, it should be stored below -4 ℃. However, in order to better ensure the stability of the product, researchers suggest that it should be configured on site during use.
Tris HCl powder of trimethylol aminomethane
Advantages of Tris HCl buffer
1. Good water solubility, high solubility in water, clear liquid;
2. High purity, up to 99%, stable performance in use;
3. Do not precipitate with some metal ions (such as calcium and magnesium);
4. The ionic strength of Tris HCl buffer is low;
5. Compared with traditional phosphate buffer, Tris HCl has better effect in enzyme determination.
Application of Tris HCl buffer
1. Tris HCl is widely used in protein, nucleic acid and cell culture buffer;
2. It can be used as a buffer for protein crystal growth;
3. Combine with glycine in the electrophoresis buffer to form a buffer system, which is used to stabilize the pH value in the electrophoresis process;
4. Due to its low ionic strength, it can be used for the formation of intermediate fibers of nematode lamin;
5. Used for titration of standard substance;
6. Used for the preparation of surfactants.
Xuzhou best Biotechnology Co., Ltd. is a manufacturer of Tris HCl buffer. The company was founded in 2016 and has been committed to the R & D and production of biological buffer for many years. It has not only established and organized its own R & D team, but also made important achievements in this field. At present, the company has produced more than 30 kinds of biological buffer products on sale, with a complete range of products. Customers with purchase intention are welcome to consult and understand!