Chinese Name: trimethylol aminomethane
Foreign name: tris (hydroxymethyl) methyl aminomethane tham
Alias: tromethamine
Chemical formula: c4h11no3
Molecular weight: 121.135
CAS Login No.: 77-86-1
ECS login number: 201-064-4
Boiling point: 357.00 ℃
Density: 1.334 g/cm³
Appearance: white crystalline particles
Flash point: 169.71 ℃
Application: biological buffer, etc
essential information
Chinese Name: trimethylol aminomethane
English Name: tris (hydroxymethyl) methyl aminomethane tham
English alias: Tris base; 2-Amino-2-（hydroxymethyl）-1,3-propanediol; THAM; Trometamol
CAS No.: 77-86-1
Molecular formula: c4h11no3
Linear formula: nh2c (CH2OH) 3
Molecular weight: 121.14
Purity: ≥ 99.5%
Grade: br
MDL No.: mfcd00004679
Beilstein No.: 741883
Character description
White crystalline particles
Physical parameters
pKa：8.1
PH application range: 7-9
Melting point: 168-172 ° C
Boiling point: 219-220 ° c/10mmhg (lit.)
quality standard
Purity: ≥ 99.5%
Insoluble matter: acceptable
Loss on drying (110 ° C): ≤ 0.2%
Chloride (CL):<0.005%<>
Sulfate (SO4):<0.005%<>
Iron (FE):<0.0005%<>
Hazard statement
Hazard Code: Xi
Hazard level: 36
Safety level: 26
Buffer
Formula: (0.5m ph7.6), tris (trimethylol aminomethane) 60.57g, 1n HCl about 420ml, double distilled water to 1000ml.
Preparation method: dissolve Tris with a small amount of double distilled water (300~500ml), add HCl, adjust the pH to 7.6 with HCl (1n) or NaOH (1n), and finally add 1000ml of double distilled water. This solution is a stock solution and is stored in a refrigerator at 4 ℃.
Note: the pH value of Tris HCl changes with temperature, so it should be measured at room temperature, so the measured results are more reliable.
Uses: Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Buffer characteristics: Tris is a weak base, and its PKA is 8.1 at room temperature (25 ℃); According to the buffer theory, the effective buffer range of Tris buffer is between pH7.0 and 9.2. The pH of Tris alkali aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer of this pH value. But at the same time, we should pay attention to the influence of temperature on the pKa of Tris.
Trimethylol aminomethane is an organic substance with the chemical formula c4h11no3 and white crystalline particles. It can be used as a biological buffer; Used for gel electrophoresis configuration buffer. As an alkaline drug, it is used to correct acidosis without increasing carbon dioxide retention. 7.28% tham 2-3ml/kg each time (isotonic solution is 3.64%), dilute it with the same amount of 5%-10% glucose solution and drip it slowly. The English abbreviation commonly used in clinic is tham.