Tris HCl, Chinese alias: trimethylaminomethane hydrochloride; Tris hydrochloride, English Name: Tris hydrochloride.
Chinese Name: trimethylaminomethane hydrochloride
Chinese alias: tris (hydroxymethyl) aminomethane; Tromethamine; Ammonium bradykinic acid; Trimethylol aminomethane; Tris hydrochloride
Foreign name: Tris HCl
English Name: tris (hydroxymethyl) aminomethane
Molecular formula: c4h11no3
Molecular weight: 157.6
CAS accession No.: 1185-53-1
Melting point: 150 to 155 ℃
Boiling point: 219 to 220 ℃ (10 mmHg)
Water solubility: 550G / L (25 ℃)
Density: 1.353 kg / M ³
Appearance: white crystal
Water solubility: colorless, clear
Flash point: 100 ℃
Application: PCR amplification of DNA.
Safety Description: low toxicity, carcinogenic, do not directly contact the skin.
Preparation method
10x tbe reservoir preparation method: dissolve 108G tris (FW = 121), 55g boric acid (FW = 61.8) and 40ml 0.5m EDTA in 600ml deionized water; Then adjust the pH to 8.3, add deionized water to volume to 1L, and store at room temperature.
Diluted 10 times during use, i.e. 1 × TBE Buffer。
Application: PCR amplification of DNA.
Main ingredients: Tris HCL buffer (0.05mol/l, 25 ℃); Hydrochloric acid; Tris solution.
Uses: Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators and some drugs. Tris was also used as titration standard. When using Tris as protein buffer, if mass spectrometry is required for subsequent work, it is not appropriate to use Tris, and it is better to replace it with a buffer that can be tolerated by other mass spectrometers.