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Chinese name: Trihydroxymethyl aminomethane
English name: Tris (hydrogen) amine
CAS registration number: 77-86-1
EINECS Login No.: 201-064-4
Molecular formula: C4H11NO3
Molecular weight: 121.14
Linear formula: NH2C (CH2OH) 3
Molecular weight: 121.14
Purity: ≥ 99.5%
Grade: BR
MDL No.: MFCD00004679
Beilstein No. 741883
EC No: 201-064-4
Specification: 25Kg
Grade: biochemical grade
Quality standard:
Appearance: white crystal
Content: ≥ 99.9%
Melting point: 168-171
Water insoluble matter: ≤ 0.03%
Strong heat residue: ≤ 0.05%
Heavy metal: ≤ 5ppm
Moisture: ≤ 0.2%
pH：10.0-10.8 pH unit
Chroma: 5#
Ultraviolet absorbance/260nm: ≤ 0.1Abs unit
Iron: ≤ 2ppm
Chloride: ≤ 3ppm
Upstream raw material: paraformaldehyde; Methanol; Trihydroxymethyl nitromethane; Nitromethane
Product application: Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris was used to grow protein crystals at different pH. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Production method:
First dissolve Tris with a small amount of double distilled water (300~500ml), add HCl, then adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and finally add double distilled water to 1000ml. This solution is a stock solution and stored in a refrigerator at 4 ℃.
Note: The PH value of Tris HCl varies with temperature, so it should be measured at room temperature, so the measured results are reliable.
Uses: Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important applications. Tris was used to grow protein crystals at different pH. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Buffer characteristics: Tris is a weak base, and its pKa is 8.1 at room temperature (25 ℃); According to the buffer theory, the effective buffer range of Tris buffer solution is between pH 7.0 and 9.2.
The pH of Tris alkali aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain the buffer solution of this pH value. However, attention should be paid to the influence of temperature on pKa of Tris.