Product Name: guanidine isothiocyanate
CAS No.：593-84-0
Molecular formula: ch5n3 CHNS
Molecular weight: 118.16
Alias: guanidine thiocyanate
Introduction of guanidine isothiocyanate
Guanidine isothiocyanate, also known as GTC and guanidine thiocyanate, is mainly used in biomedicine, chemical reagents, etc. as a liquid separation agent and a strong denaturant, it is used for cell lysis and denaturation; RNA and DNA extraction. As a DSSC adsorbent, guanidine isothiocyanate can improve the conversion of DSSC.
Guanidine isothiocyanate vs guanidine hydrochloride
Guanidine isothiocyanate and guanidine hydrochloride are both protein denaturants. Guanidine isothiocyanate is a more effective protein denaturant than guanidine hydrochloride. When intact RNA is isolated, it is usually used to eliminate RNase activity.
Guanidine isothiocyanate on skin
Guanidine isothiocyanate is harmful to human skin, so it is necessary to wear laboratory overalls during the experiment to avoid direct contact with guanidine isothiocyanate.
Guanidine isothiocyanate pollution
Guanidine isothiocyanate has little effect on a260 / A280 ratio, so it has little effect on RNA quantification. However, its presence has a great impact on the a260 / A230 ratio. When it contains 0.5% pollutants, the ratio of a260 / A230 decreases to less than 0.5. Avoid using samples containing suspected guanidine contamination, as this will adversely affect the downstream enzymatic reaction.
Solubility of guanidine isothiocyanate
2g/50ml in water
Guanidine isothiocyanate DNA extraction scheme
It is reported and studied that the phenol lithium chloride method can simultaneously extract RNA and DNA. It divides cells through phenol and SDS, denaturates and depolymerizes proteins, inhibits RNase, and protects DNA with EDTA. Finally, DNA and RNA are separated according to the different precipitation rates of RNA and DNA in Li +. The whole process is completed within 2 hours.
Extraction scheme of guanidine isothiocyanate phenol chloroform
They can jointly inhibit RNase, and the protein mutates under the joint action of guanidine isothiocyanate and sodium lauryl sarcosine, thus releasing RNA. Under acidic conditions, DNA rarely dissociates, and the same protein A denaturates and precipitates, while RNA is in the upper layer.
Packaging form of guanidine isothiocyanate
25kg/ cardboard drum
Storage method of guanidine isothiocyanate
Store in a dry, well ventilated place, away from direct sunlight.
