Trimethylolaminomethane is an organic substance with the chemical formula C4H11NO3 and white crystalline particles. It can be used as a biological buffer; Gel electrophoresis is used to configure buffer solution. * as an alkaline drug, it is used for correction of acidosis and does not cause carbon dioxide retention. Each time 7.28% tham 2-3ml / kg (isotonic solution is 3.64%), dilute it with the same amount of 5% - 10% glucose solution and drip it slowly. The English abbreviation commonly used in clinic is tham.
Trimethylolaminomethane
Foreign name: tris (hydroxymethyl) methyl aminomethane tham
Alias: aminobutanol
Chemical formula: C4H11NO3
Molecular weight: 121.135cas
Login No.: 77-86-1
EINECS login No.: 201-064-4
Boiling point: 357.00 ℃
Density: 1.334 g / cm ³
Appearance: white crystalline particles
Flash point: 169.71 ℃
Application: biological buffer, etc
essential information
Chinese Name: trimethylolaminomethane
English Name: tris (hydroxymethyl) methyl aminomethane tham
English alias: Tris base; 2-Amino-2-（hydroxymethyl）-1,3-propanediol; THAM; Trometamol
CAS No.: 77-86-1
Molecular formula: c4h11no3
Linear molecular formula: nh2c (CH2OH) 3
Molecular weight: 121.14
Purity: ≥ 5.99%
Grade: br
MDL No.: MFCD00004679
Beilstein No.: 741883
EC No.: 201-064-4
Character description
White crystalline particles
Physical parameters
pKa：8.1
PH application range: 7-9
Melting point: 168-172 ° C
Boiling point: 219-220 ° C / 10mmhg (lit.)
quality standard
Purity: ≥ 99.5%
Insoluble matter: acceptable
Loss on drying (110 ° C): ≤ 0.2%
Chloride (CL): < 0.005%
Sulfate (SO4): < 0.005%
Iron (FE): < 0.0005%
Hazard statement
Hazard Code: Xi
Hazard level: 36
Safety level: 26
Buffer
formula
（0.5M pH7.6）
Tris (trimethylolaminomethane) 60.57g
1n HCl about 420ml
Add double distilled water to 1000ml
Manufacturing method
Dissolve Tris with a small amount of double distilled water (300 ~ 500ml), add HCl, adjust the pH to 7.6 with HCl (1n) or NaOH (1n), and finally add double distilled water to 1000ml. This solution is a stock solution and stored in a refrigerator at 4 ℃.
Note: the pH value of Tris HCl changes with the change of temperature, so it should be measured at room temperature, so the measured results are more reliable.
purpose
Tris buffer is widely used not only as a solvent for nucleic acids and proteins, but also for many important purposes. Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Buffer characteristics
Tris is a weak base, and its PKA is 8.1 at room temperature (25 ℃); According to the buffer theory, the effective buffer range of Tris buffer is pH7 Between 0 and 9.2.
The pH of Tris alkali aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer of this pH value. However, attention should be paid to the effect of temperature on pKa of Tris.