Trimethylolaminomethane is an organic substance with the chemical formula C4H11NO3 and white crystalline particles. It can be used as a biological buffer; Gel electrophoresis is used to configure buffer solution. As an alkaline drug, it is used for correction of acidosis and does not cause carbon dioxide retention. Each time 7.28% tham 2-3ml / kg (isotonic solution is 3.64%), dilute it with the same amount of 5% - 10% glucose solution and drip it slowly. The English abbreviation commonly used in clinic is tham.
Manufacturing method
Dissolve Tris with a small amount of double distilled water (300 ~ 500ml), add HCl, adjust the pH to 7.6 with HCl (1n) or NaOH (1n), and finally add double distilled water to 1000ml. This solution is a stock solution and stored in a refrigerator at 4 ℃.
Note: the pH value of Tris HCl changes with the change of temperature, so it should be measured at room temperature, so the measured results are more reliable.
Purpose
Tris buffer is widely used not only as a solvent for nucleic acids and proteins, but also for many important purposes. Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Buffer characteristics
Tris is a weak base, and its PKA is 8.1 at room temperature (25 ℃); According to the buffer theory, the effective buffer range of Tris buffer is pH7 Between 0 and 9.2.
The pH of Tris alkali aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer of this pH value. However, attention should be paid to the effect of temperature on pKa of Tris.