Summary:
Basic information: Tris
Chinese Name: trimethylolaminomethane
English Name: tris (hydroxymethyl) methyl aminomethane tham
English alias: 2-amino-2 - (hydroxymethyl) - 1,3-propanediol; THAM； Trometamol。
CAS No.: 77-86-1
EC No.: 201-064-4
Molecular formula: C4H11NO3
Linear formula: NH2C (CH2OH) 3
Molecular weight: 121.14
Purity: ≥ 99%
Description: white crystalline particles
pKa：8.1
PH application range: 7-9
Melting point: 168-172 ° C
Boiling point: 219-220 ° C / 10mmhg (lit.)
Others: soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride. Solubility at 25 ° C (mg / ml): water 550, ethylene glycol 79.1, methanol 26, absolute ethanol 14.6, 95% ethanol 22.0, N, N-dimethylformamide 14, pentanol 2.6, propanol 2.0, ethyl acetate 0.5, cyclohexane 0.1. The aqueous solution does not absorb carbon dioxide, which has corrosive effect on copper and aluminum and is irritating. It is the solvent of nucleic acid and protein and should be widely used It is used for the preparation of buffer in biochemistry and molecular biology experiments.
Purpose:
1.1m Tris HCl 6.8, 1.5m Tris HCl 8.8 and 5 × Tris glycine electrophoresis buffer is the most commonly used reagent in SDS-PAGE.
2. Add EDTA into Tris HCl buffer to make "Te buffer", which is used for DNA stabilization and storage. "Tae (Tris / acetate / EDTA) buffer" can be obtained by replacing hydrochloric acid regulating pH value with acetic acid, and "tbe (Tris / borate / EDTA) buffer" can be obtained by replacing boric acid. TAE and tbe buffers are often used for nucleic acid electrophoresis the test is in progress.
3. Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans.
4. * used as a pharmaceutical intermediate, as an alkaline drug, for the correction of acidosis and does not cause carbon dioxide retention.
Each time 7.28% tham 2-3ml / kg (isotonic solution is 3.64%), dilute it with the same amount of 5% - 10% glucose solution and drip it slowly. The English abbreviation commonly used in clinic is tham.
5. The intermediate of fosfomycin can also be used as vulcanization accelerator, cosmetics (cream and lotion), mineral oil and paraffin emulsifier.
Storage conditions: dry at room temperature.
Common Tris buffer system:
T1010 1.5M Tris-HCL（pH8.8）
T1020 1M Tris-HCL（pH6.8）
T1090 1M Tris-HCL（pH=7.4）
T1130 1M Tris-HCl（pH=7.0）
T1140 1M Tris-HCl（pH=7.5）
T1150 1M Tris-HCl（pH=8.0）
T1160 1M Tris-HCl（pH=9.0）
T1050 5 × Tbe buffer
T1051 10 × Tbe buffer
T1060 50 × Tae buffer
T1070 5 × Tris glycine electrophoresis buffer
T1510 10x Tris mops buffer
T1510 10x Tris mops buffer
T1120 te buffer, pH = 8.0.
synthesis:
Method 1: a preparation method of trimethylolaminomethane. The specific preparation steps are as follows:
(1) Adding trimethylolmethane into methanol aqueous solution, heating to 50-70 ℃, stirring and dissolving, wherein the mass volume ratio of trimethylolmethane to methanol aqueous solution is 8:3-7 according to g / ml, and the methanol aqueous solution is prepared by mixing purified water and methanol according to the volume ratio of 2:3;
(2) Adding charcoal activated carbon to the solution, wherein the weight ratio of charcoal activated carbon to trimethylolmethane is 0.5-2:100, holding at 45-55 ℃ for 20-40 minutes, filtering while hot and collecting the filtrate;
(3) The filtrate is concentrated under reduced pressure at a concentration temperature of 70-80 ℃ until crystallization occurs, and then placed in a cool place;
(4) After the crystallization is separated by suction filtration, it is washed with absolute ethanol and dried at 40-60 ℃ for 3-5 hours.
According to the above preparation method of trihydroxymethylaminomethane, the purity of trihydroxymethylaminomethane is high, which meets the demand of reference reagent for the purity of trihydroxymethylaminomethane, and the process is stable and reasonable. It is especially suitable for the production of kilogram batch products. The process is simple and reasonable, the product qualification rate is high, and it is suitable for the needs of large-scale industrial production.
Method 2: a synthetic method of trihydroxymethylaminomethane, which uses nitromethane and excess paraformaldehyde as main raw materials and condenses to trihydroxymethylnitromethane at a certain temperature; Then, under certain temperature, pressure, solvent and catalyst, trimethylolaminomethane is prepared by hydrogenation reduction reaction, crystallization and recrystallization.
The condensation temperature is 27 ~ 60 ℃, the reduction temperature is 25 ~ 60 ℃, the pressure is 0.1 ~ 4MPa, the catalyst is nickel catalyst, the solvent is methanol, and the molar ratio of nitromethane to paraformaldehyde is 1∶3 ~ 4.
Compared with the original synthesis method of trimethylolaminomethane, the invention adopts nitromethane and excess paraformaldehyde as the main raw materials, and the consumption of nitromethane is 0.85 T / T; The price of paraformaldehyde is 5000 yuan / ton, and the material consumption is 1.1 tons / ton; Methanol consumption is 1.6 T / T. This method consumes less materials and has low manufacturing cost, which is 25000 ~ 30000 yuan / T, which is 66% of the original synthesis method. In addition, the recrystallization process of the product adopts water refining, with good product quality, content of 99.94% and absorbance (260nm) of 0.05; The methanol (more than 95%) obtained by distillation of the mother liquor in the crystallization process can be used for reduction reaction with less three wastes. Therefore, the production cost of trimethylolaminomethane prepared by this method is low, the product quality is good and there are less three wastes.