Product introduction:
Ribonuclease A is an endonuclease that can specifically attack RNA The 3 'end of the pyrimidine residue cleaves the phosphate diester bond formed with the adjacent nucleotide. The end products of the reaction are pyrimidine 3 'phosphate and oligonucleotides with pyrimidine 3' phosphate at the end. In the absence of cofactors and divalent cations, the effect of ribonuclease A can be inhibited by placental RNase inhibitors or oxyvanadium ribonucleoside complexes.
Product application:
Biochemical research; Determining the structure of nucleic acid; Ribonucleic acid (RNA) sequence analysis; RNA in hydrolyzed protein samples; Purified DNA.
Usage method:
DNase free RNase A can be prepared by boiling. First, RNase A was dissolved in 10mmol / L Tris HCl (pH 7.5) and 15 mmol / L NaCI to prepare a concentration of 10mg / ml, heated at 100 ° C, kept for 15 minutes, slowly cooled to room temperature, and stored at - 20 ° C after sub packaging. (Note: the configuration method is only for reference. Please use it according to the specific experimental requirements and the literature.)
Matters needing attention:
1. This product is only for scientific research. Do not use for medicine, clinical diagnosis or treatment, food and cosmetics.
2. For your safety and health, please wear experimental clothes and disposable gloves.
Introduction: ribonuclease A is an endonuclease that can specifically attack the 3 'end of pyrimidine residue on RNA and cleave the phosphate diester bond formed with adjacent nucleotides.
Application: biochemical research; Determining the structure of nucleic acid; Ribonucleic acid (RNA) sequence analysis.